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D-Fructose-6-phosphate + ATP -> D-Fructose-1,6-bisphosphate + ADP + H+
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/molGlycolysis step 2: Isomerization of glucose-6-phosphate catalysed by Phosphoglucoisomerase:α-D-Glucose-6-phosphate <=> D-Fructose-6-phosphateδGo = +1.7 kJ/molGlycolysis step 3: Second phosphorylation catalysed by Phosphofructokinase:<span>D-Fructose-6-phosphate + ATP -> D-Fructose-1,6-bisphosphate + ADP + H+δGo = -18.5 kJ/molGlycolysis step 4:Cleavage to two Triose phosphates catalysed by Aldolase: D-Fructose-1,6-bisphosphate <=> Dihydroxyacetone phosphate + D-glyceroaldehyde-3-phosph


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