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D-glyceroaldehyde-3-phosphate + NAD+ +Pi <=> 1,3-Bisphosphoglycerate + NADH + H+
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oxyacetone phosphate <=> D-glyceroaldehyde-3-phosphate╬┤Go = +7.6 kJ/molGlycolysis: Energy generation phase3-BisphosphoglycerateGlycolysis step 6:Generation of 1,3-Bisphosphoglycerate catalysed by Glyceraldehyde-3-phosphate dehydrogenase:<span>D-glyceroaldehyde-3-phosphate + NAD+ +Pi <=> 1,3-Bisphosphoglycerate + NADH + H+╬┤Go = +6.3 kJ/mol 3-PhosphoglycerateGlycolysis step 7:Substrate-level phosphorylation, 3-Phosphoglycerate catalysed by Phosphoglycerate kinase:1,3-Bisphosphoglycerate + ADP <=> 3-P


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