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2-Phosphoglycerate <=> Phosphoenolpyruvate + H2O
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18.8 kJ/molGlycolysis step 8:Phosphate transfer to 2-Phosphoglycerate catalysed by Phosphoglycerate mutase:3-Phosphoglycerate <=> 2-PhosphoglycerateδGo = +4.4 kJ/molGlycolysis step 9:Synthesis of Phosphoenolpyruvate catalysed by Enolase:<span>2-Phosphoglycerate <=> Phosphoenolpyruvate + H2OδGo = +1.7 kJ/molGlycolysis step 10:Substrate-level phosphorylation. Pyruvate synthesis catalysed by Pyruvate kinase:Phosphoenolpyruvate + H+ + ADP -> Pyruvate + ATPδGo = -31.4 kJ/mol


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