ΔGs of a pathway are additive: This additive property of free energy changes is very important in biochemical pathways through which substrates must pass in a particular direction (for example, A → B → C → D → ...). As long as the sum of the ΔGs of the individual reactions is negative, the pathway can potentially proceed as written, even if some of the individual reactions of the pathway have a positive ΔG. The actual rate of the reactions does, of course, depend on the lowering of activation energies by the enzymes that catalyze the reactions.