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#Biochemistry #voets
Bacteria can produce eukaryotic proteins only if the recombinant DNA that carries the protein-coding sequence also includes bacterial transcriptional and translational control sequences. Synthesis of eukaryotic proteins in bacte- ria also presents other problems. For example, many eukaryotic genes are large and contain stretches of nucleotides (introns) that are transcribed and excised before translation (Section 26-3A); bacteria lack the machinery to excise the introns. In addition, many eukaryotic proteins are posttranslationally modi- fied by the addition of carbohydrates or by other reactions. These problems can be overcome by using expression vectors that propagate in eukaryotic hosts, such as yeast or cultured insect or animal cells.
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owner: smelly_compost - (no access) - Voet's Fundamentals of Biochemistry 4th Edition.pdf, p102


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